The determination of phenolic compounds in artichoke flower head extracts by HPLC GC/MS technique

Abstract

AIM: The aim of the research was the study of component composition of artichoke extract and its standardization. MATERIALS AND METHODS: Dry artichoke flower heads (inflorescences) was the plant raw material which was selected as the object of research. Methanol extracts and methanol extracts after hydrolysis were analyzed by HPLC GC/MS technique. RESULTS AND CONCLUSIONS: As a result of the research, eight compounds such as luteolin-7-glucoside, luteolin, apifenin-7-glucoside, hyperoside, rutin, caffeic acid, chlorogenic acid and rosmarinic acid were detected in the tested solutions. Four phenolic compounds such as apigenin-7-glucoside, luteolin, chlorogenic acid and luteolin-7-glucoside were identified in the studied methanol extract after hydrolysis. HPLC technique is the validated quality control technique of the obtained extract.