Химико-токсикологический анализ сертралина в биологическом материале

Abstract

Установлена степень изолирования сертралина из биологического материала с помощью общепринятых в химико-токсикологическом анализе методов. Наиболее эффективным оказался метод А.А. Васильевой, с помощью которого выделено 20,8±1,9% сертралина. Идентификацию и количественное содержание сертралина в элюатах проводили методом ВЭЖХ с мультиволновым УФ-детектированием.

Sertraline, an antidepressant of the selective serotonin reuptake inhibitor class, one of the most potent of reversible serotonin inhibitors, is used to treat severe depressions, but it was on repeated occasions a cause of fatal poisoning. The trial established the degree of sertraline isolation from biological material, by using the universally accepted chemotoxicological methods described by A.A. Vasilyeva, V.F. Kramarenko, and Stas-Otto. Sertraline was found in the alkaline-chloroform extracts that had been previously subjected to additional extraction and TLC purifications. Sertraline eluates were analyzed by HPLC and multi-wave UV detection. The drug was identified from its retention time (tR = 23.32±0.01 min) and spectral characteristics; LOD = 5 μg/ml (criterion, Ss/Sn = 3:1 for λ = 280 nm). The quantity of sertraline in the eluates was determined using the calibration ratio of the area of the chromatographic peak (Y) to the concentration (X) (detected at a wavelength of 280 nm), which was described by the equation: Y = (1.75•10-4 ± 3•10-6) × (r = 0.999; So 2 = 3•10-8). There were some validation characteristics of the methods: linearity was observed in the range from 12.2 to 100 μg/ml; LOQ = 12.2 μg/ml (criterion, 10σ). The correctness and accuracy of the procedure were (n = 5): the range of low (98.9%, RSD = 1.6%); moderate (100.8%; RSD = 1.4%), and high (100.5%, RSD = 1.0%) concentrations. Sertraline was isolated from the liver in a quantity of 20.8±1.9% by the Vasilyeva method, 11.1±1.4% by Stas-Otto method, and 14.8±1.8% by the Kramarenko method. The methods to isolate sertraline from biological material should be further optimized using amphiphilic or lipophilic extragents.