Antimicrobial and antioxidant activity of anthocyanin complexes of some berries’ species of Ukraine

Abstract

Antibacterial drugs often become the basis for the treatment of infectious diseases. It is known that the potential of anthocyanin extracts is considered to be natural effective alternative antimicrobial agents. In addition, the ability of extracts to decrease adhesion without reducing the bacterial growth diminishes the probability of developing resistance while reducing the risk of infection. The aim of the study was to determine the degree of antimicrobial activity of the anthocyanin complexes of Aronia melanocarpa (Aronia melanocarpa), black currant (Ribes nigrum), elderberry (Sambucus nigra) with reference culture Staphylococcus aureus ATCC 25923, Escherichia coli АТСС 25922, Bacillus subtilis ATCC 6633, Pseudomonas aeruginosa АТСС 27853, Candida albicans NСTC 885-653. The highest degree of microbiological sensitivity of bacterial cultures Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa was observed in the native form of anthocyanin complexes derived from black currant (Ribes nigrum), Escherichia coli and Bacillus subtilis – to the anthocyanins in native form, derived from elder black (Sambucus nigra), which indicates their high antimicrobial activity against the microorganisms mentioned above. Reducing the concentration of anthocyanins in alcoholic extract at dilution in the ratio of 1:1 and 1:2 significantly decreased the microbiological sensitivity of microorganisms to them Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa. When surveying the effects of anthocyanin complexes from Aronia melanocarpa, Ribes nigrum, Sambucus nigra on Candida albicans fungi culture, no sensitivity was established, which indicates the absence of antimycotic activity of the above complexes in laboratory conditions in vitro. Anthocyanin complexes obtained from Aronia melanocarpa, Ribes nigrum, Sambucus nigra are complicated in terms of their chemical composition compounds for which the integral value of antioxidant activity extracts of the samples under study is a quantitative estimate of the total content of the bioflavanoid that determines their antimicrobial activity in vitro.