Please use this identifier to cite or link to this item: http://dspace.nuph.edu.ua/handle/123456789/21066
Title: The immunomodulatory activity of the extracts and complexes of biologically active compounds of Galium verum L. herb
Other Titles: Imunomodulační aktivita extraktů a komplexů biologicky aktivních látek Galium verum L. herba
Authors: Shinkovenko, I. L.
Kashpur, N. V.
Ilyina, T. V.
Kovalyova, A. M.
Goryacha, O. V.
Koshovyi, O. M.
Toryanyk, E. L.
Kryvoruchko, O. V.
Шинковенко, И. Л.
Шинковенко, І. Л.
Кашпур, Н. В.
Ильина, Т. В.
Ільїна, Т. В.
Ковалева, А. М.
Ковальова, А. М.
Горячая, А. В.
Горяча, О. В.
Кошевой, О. Н.
Кошовий, О. М.
Торяник, Е. Л.
Торяник, Э. Л.
Криворучко, О. В.
Keywords: Galium verum L.;immunomodulatory activity;lymphocyte blast transformation;fluid water extract;polysaccharide complex;polyphenols complex
Issue Date: 2018
Bibliographic description (Ukraine): The Immunomodulatory Activity of the Extract and Complexes of Biologically Active Compounds of Galium verum L. herb / I. L. Shinkovenko, N. V. Kashpur, T. V. Ilyina, A. M. Kovalyova, O. V. Goryacha, O. M. Koshovyi, E. L. Toryanyk, O. V. Kryvoruchko // Ceska a Slovenska Farmacie. - 2018. - № 67 (1). - Р. 25-29.
Abstract: The present article discusses the results of an immunomodulatory activity of Galii veri herba, Galium verum herb (Galium verum L., Rubiaceae) fluid water extract and the effect of the polysaccharide and phenolic complexes of the letter on the immunomodulatory activity. The same extract was fractionated into a polysaccharide complex (PSC) and a polyphenolic complex (PPC). In the obtained substances, the contents of hydroxycinnamic derivates, flavonoids and polyphenols were determined spectrophotometrically; polysaccharides were quantified gravimetrically; the immunomodulatory activity of the substances was determined in the reaction of lymphocyte blast transformation. It has been established that the fluid extract of Galium verum herb contains 6.3% polysaccharides, 4.2% hydroxycinnamic derivates expressed as chlorogenic acid, 0.4% flavonoids expressed as rutin, 3.7% polyphenols expressed as gallic acid. PPC contains 4.48% hydroxycinnamic derivates expressed as chlorogenic acid, 0.43% flavonoids expressed as rutin, and 3.95% polyphenols expressed as gallic acid. The lowest immunomodulatory activity was found for PPC. A significantly higher activity was determined for PSC. The highest immunomodulatory activity was established for the fluid extract at a dilution of 1/20, its activity being by 59.4% higher compared with the lymphocyte spontaneous transformation and by 18.5% higher than that of the reference substance PHA. The obtained results enable an assumption of a synergistic effect of PPC and PSC of Galium verum herb fluid extract on the potency of its immunomodulatory activity.
URI: http://dspace.nuph.edu.ua/handle/123456789/21066
Appears in Collections:Наукові публікації кафедри фармакогнозії

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