Зміна концентрації лептину в оваріектомованих щурів із метаболічним синдромом за умов введення сполук з імуномодулюючою активністю

Abstract

Было исследовано влияние соединений с иммуномодулирующими свойствами левамизола производного левамизола-308 (ПЛ-308) на уровень лептина в овариэктомованих крыс с метаболиче-ским синдромом (МС). Индукцию МС начинали через 2 недели после хирургического вмешательства пу-тем удержания животных на высокоуглеводной диете, которая обеспечивалась свободным доступом крыс до 30% раствора сахарозы в качестве питьевой воды в течение 5 недель. Установлено, что гипоэстрогения привела к снижению уровня лептина. При сочетании дефицита эстрогенов с МС уровень лептина оставался таким же низким, как и в овариэктомованих крыс без МС. Введение левамизола и ПЛ-308 способствовало повышению уровня лептина, что свидетельствует об улучшении метаболического состояния животных фоне имуномодуляции.

Today the role of leptine in modulating methabolic, energetic and immune effects is actively stud-ied. The effect of compounds with immunomodulating properties on the course of the metabolic syndrome (MS) in combination with hypoestrogeny has not been sufficiently studied today. In the laboratory of hormone-like compounds synthesis of the State Institution “V. Danilevsky Institute for Endocrine Pathology Problems of Na-tional Academy of Medical Science of Ukraine” (Kharkіv, Ukraine) we have synthetically produced the derivative of levamisol-308 (PL308) which corresponds to the class 5 of toxicity (almost non-toxic compounds) according to the acute toxicity data, when the levamisol corresponds to the class 4 (low-toxic compounds). The purpose of research is to investigate the effect of levamisole and its derivative PL-308 on the leptin con-centration in rats with MS against a background of estrogen deficiency. The object and methods of research. The tests are made on the 3-month white female rats of the line Vistar. We reproduced the hypoestrogenia by the way of two-side overiaectomia with the light etherization. We preceded the induction of MS within 2 weeks aftert of the operative intervention. The high-carbohydrate diet (HCD) was provided by the free access to the 30 % sweet water as a potable water during 5 weeks. Insulin resistance was determined by the parameters of the area under the glycemic curve, body weight and leptin level (by ELISA method) in the ovariectomized female rats with MS in conditions of administration of com-pounds with immunomodulatory properties such as levamisole and its derivative PL-308. The compounds were administered for 5 weeks by the means of a zonde. Results and discussion. The combination of the hypoestrogenia with the MS causes the carbohydrate intolerance and body mass increase with the animals. The administration of immunomodulatory compounds didn`t influence on the animals body mass but normalized the square of glycemic curve. The hypoestrogenia causes the decrease of the leptine level. The combination of the estrogen deficit with MS doesn`t influence the index further. The administration of levamisole and PL-308 has promoted the increase of the leptine level. Conclusions. The levamisol and its derivative PL-308 have improved the metabolic conditions with the animals which is proved by the leptine level increase and the carbohydrates intolerance decrease.