Research of Vitis Vinifera leaves extraction process on timatic laboratory extractor

Abstract

Herbal medicinal products are widely used for prevention and treatment of many diseases. AIM: The aim of our work was to study the parameters of grape leaves extraction on a laboratory extractor Timatic Micro. MATERIALS AND METHODS: Towards achieving the goal, the tasks of determining the following factors influencing the grape leaves extraction were set: the degree of grinding and extraction time. Timatic Micro extractor was used for extraction. This contemporary technology is based on a double action of pressure – reduction of the pressure and percolation of biological material that intensifies extraction process. The extractant used was 40% ethyl alcohol as it provides maximum extraction of flavonoids and tannins. The extractor was set with standard parameters: compression - 2 minutes, decompression - 2 minutes. RESULTS AND DISCUSSION: The degree of raw material depletion in the main biologically active substances of common grape leaves (tannins and flavonoids) significantly increases with decreasing the size of crushed grape leaves, and is the highest in the fraction less than 1 mm and is 69.93 %, 44.13% and 78.48% for extractives, tannins, and flavonoids, respectively (extraction time - 2 h). With increasing the time of extraction up to 4 h the yield of extractible substances increases by an average of 3%, but for 2 hours quite intensive extraction occurs, which gives high rates of raw material depletion. For obtaining the extract in the laboratory extractor Timatic, we have chosen as raw material a fraction of grape leaves of less than 1 mm and extraction time of 2 hours. CONCLUSION: The presented method has proven its reliability and efficiency in the extraction of substances in question.