Спосіб полярографічного визначення фамотидину з використанням реагенту оксону

Abstract

A new polarographic technique for the determination of famotidine is proposed, which is based on the polarographic reduction of its derivative obtained with potassium peroxymonosulfate. The proposed method for sensitivity and selectivity outweighs the existing voltammetric methods for the determination of famotidine, and is more expressive and economically more advantageous than chromatographic techniques. Using fast-scanning polarography, we found that the famotidine derivative is restored within a pH of 3.0 to 11.0. The peak current of recovery of famotidine derivative reaches its maximum value at pH 8.5. The optimal conditions for the process of derivatization of famotidine by means of peroxymonosulfate potassium were investigated, and hence the polarographic determination of it on the year. Optimal oxidation conditions: pH in the range of 8.0–9.0; not less than twice the excess of the KPMS; oxidation time of at least 10 minutes at 80 ºC. Against the background of a phosphate buffer solution of pH 8.5, the famotidine derivative is recovered to form a characteristic peak at –1.4 V. Depending on the rate of polarizing voltage, the absorption nature of the current is indicated. The concentration dependence of the peak heights in the range of 2•10-5–2•10-4 mol/L is linear (r = 0.9990), which allows it to be quantified for famotidine. On the basis of the obtained results, a method of quantitative polarographic determination of famotidine in Famotidine tablets (Arterium Corporation, Ukraine) was developed. The proposed methodology can be used to develop analytical regulatory documentation for a medicinal product. The electrochemical process of derivative reduction is one-electron.