Phytochemical profile and pharmacological activity of the dry extract from Arctostaphylos uva-ursi leaves modified with phenylalanine

Abstract

The object of the study was dry extract from the leaves of Arctostaphylos uva-ursi, modified with phenylalanine. Phytochemical studies of phenolic compounds and saponins were carried out by HPLC and spectrophotometry. Determination of diuretic activity was carried out according to the method of E.B. Berkhina on outbred rats. Determination of the anti-inflammatory activity of the obtained extracts was carried out by the method of carrageenan edema in white rats. The study of the antibacterial activity of the extracts was carried out by the method of diffusion into agarIn the extracts obtained by HPLC, phenol glycoside (arbutin), 2 phenol carboxylic acids (gallic and ellagic), 6 flavonoids, 8 saponins were identified and their quantitative content was determined. Among flavonoids, hyperoside and catechin were dominant; among saponins, ursolic acid, uvaol, and lupeol were dominant. In the obtained extracts, the content of the main groups of phenolic compounds was determined by spectrophotometry. Dry extract of Arctostaphylos uva-ursi, modified with phenylalanine, showed pronounced diuretic, antiinflammatory and antimicrobial (in relation to St. aureus, E. coli, P. vulgaris, P. aeruginosa, B. subtilis and C. albicans) activity. The chemical composition, diuretic, antimicrobial and anti-inflammatory activity of dry extract from Arctostaphylos uva-ursi leaves modified with phenylalanine were determined. The obtained dry extract is noted for better solubility, bioavailability and pharmacodynamics, therefore it is a promising substance for creating new drugs in various dosage forms.