Studies of cytotoxic activity of substances obtained from leaves and roots of Sweet Flag (Acorus calamus L.) on human hepatocellular carcinoma (HеpG2) in vitro

Abstract

“In vitro” studies play a very significant role in studies of modern toxicology. This type of researches reduces the time and cost of preliminary studies, it can be easily reproduced, it allows to introduce accurately the amount of studied substances entering the cell, and most importantly – it may become an alternative to using animals in further researches. The Replacement principle means that the research is carried out on the cell cultures, tissues, etc., using computer models without conducting experiments on animals. Studying basic cytotoxicity plays a very important role in most researches conducted worldwide. The toxicity could be identified by damaging the cell membranes and/or causing dysfunctions of mitochondria.