Antioxidant and anti-cytolytic activity of parsnip (Pastinaca sativa L.) herb thick extract in conditions of catecholamine myocardiodystrophy in rats

Abstract

The aim: to study the antioxidant and anticytolytic activity of the parsnip herb thick extract (PHTE) in conditions of catecholamine myocardial dystrophy in rats. Materials and methods. Catecholamine myocardial dystrophy was reproduced in rats by a single subcutaneous injection of 0.18 % adrenaline hydrochloride solution at a dose of 0.5 mg/kg (0.28 ml/kg) of body weight. The experiment used 36 white male rats with an initial weight of 220–250 g, 4–4.5 months of age. The tested drugs were PHTE at doses of 100 and 200 mg/kg and the reference drug (RD) “Tricardin” were administered intragastrically in a therapeutic and prophylactic regimen for 7 days, the last time on the day of the experiment 1 hour before the administration of cardiotoxin. The activity of the studied agents was assessed by the survival rate of the animals, the functional state of the myocardium (ECG studies), by the effect on the heart mass ratio (HMR), the activity of aspartate aminotransferase (ASAT), the level of active products interacting with thiobarbituric acid (TBA-AP) and the content of reduced glutathione (RG) in the heart homogenate. Results. The study of the effect of PHTE on the lipid peroxidation / antioxidant protection (LPO / AOP) system showed that a pronounced antioxidant effect of the tool was found at a dose of 200 mg/kg: there was an increase in VG at the trend level (0.05<p<0.10) and a significant decrease (p<0.05) the content of TBK-AP in 1.85 times compared with the group of positive control (PC). A similar effect was found in the comparison drug “Tricardin”. PHTE at a dose of 100 mg/kg was less influential. The use of PHTE in both doses and the comparison drug “Tricardin” reduced the severity of cytolytic processes: the activity of the enzyme AST – a marker of cardiocytolysis, was lower than in the PC group. PHTE at doses of 100 and 200 mg/kg contributed to a certain normalization of some ECG parameters compared to PC: a slight insignificant decrease in heart rate and a significant decrease (p<0.05), QT interval, systolic index (SI) and decreased ischemia marker – ST segment displacement. The comparison drug "Tricardin" showed a similar effect to PHTE on the functional state of the myocardium in adrenaline intoxication. Conclusions. Parsnip herb thick extract exhibits antioxidant and anticytolytic activity, improves the functional state of the myocardium, prevents a decrease in the severity of alterative processes in the organ under conditions of adrenaline myocardial dystrophy Keywords: antioxidant and anticytolytic activity