Some remarks on flavonoid quantitation in rhododendron tomentosum

Abstract

Flavonoids are a group of phenolic compounds with various bioactivities and are widely distributed in plants. In various in vitro and in vivo models, they have exhibited diverse biological activities including anti-inflammatory, antiatherosclerotic, antitumor, antithrombogenic, antiosteoporotic, and antiviral effects [1]. Rhododendron tomentosum is considered by scientists primarily as an ethereal medicinal plant, but it also contains flavonoids. A common method for quantitative determination of the total flavonoid content in plant raw materials is the spectrophotometric method, based on the reaction of flavonoids with aluminum chloride [2,3]. The calculation is carried out on a standard substance of flavonoids. For quantitatively determining flavonoids, it is important to determine the appropriate standard substance (marker). In the case of using an incorrect standard, the results of quantitative calculations of the flavonoid content may be overestimated or, conversely, underestimated