Standardization of Calamus CO2-extract and study of individual fractions of herbal preparations

Abstract

This paper is devoted to the study of dense extract of calamus rhizomes (Acorus calamus L.), obtained in low-temperature subcritical extraction regimen by liquefied carbon dioxide from air - dry raw material at a pressure of 70 atm and a temperature range up to 30.5°C. There was determined chemical composition of the CO2-Calamus extract, obtained in subcritical conditions. As part of the extract by gas chromatography - mass spectrometry there were revealed 40 individual substances, including various structures terpenes, ethers, aliphatic aldehydes, carboxylic acids and sterols. The most characteristic groups are camphor, linalool, otsimen and pinene. Therapeutic antiinflammatory, analgesic and antiseptic effect of CO2-extract of Calamus was experimentally confirmed, that due to the large content of essential oils that are represented as the main active components of terpenes.