Screening study of anti-inflammatory and psychoactive properties of the derivatives of 3-benzyl-2-thio-5-methyl-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-6-carboxylic acid

Abstract

The anti-inflammatory activity for the promising according to PASS prediction 2-mercapto-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-6-carboxylic acid derivatives was studied using carrageenan-induced rat paw edema. In order to prove the mechanism of pharmacological action the modeling of the studied compounds binding with the COX-1 and COX-2 using Autodock Vina program was performed. The computer calculations showed that all of the compounds do not interact effectively with both isoforms of cyclooxygenase. These calculations confirmed the absence of anti-cyclooxygenase mechanism of the tested compounds action. No influence of the synthesized compounds on the central nervous system (locomotor, exploring reactions and emotional reactivity) was ascertained using the open field test.