Heteroside – 321 hepatoregeneration activity in cases of hepatitis induced by local hyperthermia

Abstract

Abstract: Lipid metabolism and proliferative activity of rat hepatocytes after induction of hepatitis by local hyperthermia and the effect of heteroside-321 on collagen contents and the rate of DNA synthesis in liver and spleen cells in animals with induced hepatitis were studied. The proliferation rate was determined using radioactive precursors of 3H-thymidine, and the rate of lipid synthesis was assessed by incorporation of the radioactive 14C sodium acetate. Separation of lipids into fractions was carried out by the thin-layer chromatography. The collagen content was determined by the concentration of hydroxyproline, and the functional activity (and restoration of the functional activity of liver?) was determined by the duration of hexenal sleep. It was shown that local hyperthermia was accompanied by an increase in the content of collagen in the liver by 55%, and the content of neutral lipids in the liver and their metabolism also increased. Along with these processes, the antitoxic function of the liver decreased, as evidenced by an increase in the duration of hexenal sleep. Intraperitoneal administration of Heterozide-321 to animals with hepatitis significantly increased the rate of the processes of regeneration in the liver 48 hours after the administration. Therefore, the heterozides can be used as activators of regeneration processes