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Title: | Phytochemical study and determination of pharmacological activities of cherry shoots dry extract |
Authors: | Lenchyk, L. V. Ленчик, Л. В. Shapoval, O. M. Шаповал, О. М. Шаповал, О. Н. Kyslychenko, V. S. Кисличенко, B. C. |
Keywords: | cherry;extract;flavonoids;sugars;hydroxycinnamic acids;anti-inflammatory activity;acute toxicity;вишня;екстракт;флавоноїди;цукри;гідроксикоричні кислоти;протизапальна активність;гостра токсичність |
Issue Date: | 2016 |
Bibliographic description (Ukraine): | Lenchyk, L. V. Phytochemical study and determination of pharmacological activities of cherry shoots dry extract / L. V. Lenchyk, O. M. Shapoval, V. S. Kyslychenko // ScienceRise: Pharmaceutical Science. - 2016. - № 1 (1). - Р. 40-45. |
Abstract: | Aim. The aim of our research was investigation of phenolic constituents, free and connected sugars of the cherry
shoots dry extract by the HPLC method and determination of acute toxicity and anti-inflammatory activity of the
extract.
Methods. Qualitative composition and content of phenolic compounds and sugars in the cherry shoots dry extract were studied by HPLC, with the Agilent Technologies chromatograph (model 1100). The acute toxicity was
studied after single intragastric administration of cherry shoots dry extract to rats. Studying the influence of the
cherry shoots dry extract during experimental inflammation exudative mechanism was carried out on a model of
acute carrageenan-induced inflammation in white rats.
Results. As a result of the study 9 phenolic compounds as derivatives of hydroxycinnamic acids and flavonoids and
5 sugars were determined. it was established the absence of toxicity of the cherry shoots dry extract in a dose 5000
mg/kg and according to toxicological classification of substances by К.К.Sidorov the cherry shoots dry extract after
intragastric administration belong to V toxicity class – practically harmless substances. Cherry shoots dry extract
showed the best anti-inflammatory effect in a dose of 100 mg/kg; it was about 36 % on average for 5 hours.
Conclusions. Considering results of chemical analysis and determination safety and antiinflammatory activity
we can assume that the cherry shoots dry extract can demonstrate analgesic and antiallergic effect, so it is
promising for further study in order to create a new effective and safe drugs for use in medical practice. Aim. The aim of our research was investigation of phenolic constituents, free and connected sugars of the cherry shoots dry extract by the HPLC method and determination of acute toxicity and anti-inflammatory activity of the extract. Methods. Qualitative composition and content of phenolic compounds and sugars in the cherry shoots dry extract were studied by HPLC, with the Agilent Technologies chromatograph (model 1100). The acute toxicity was studied after single intragastric administration of cherry shoots dry extract to rats. Studying the influence of the cherry shoots dry extract during experimental inflammation exudative mechanism was carried out on a model of acute carrageenan-induced inflammation in white rats. Results. As a result of the study 9 phenolic compounds as derivatives of hydroxycinnamic acids and flavonoids and 5 sugars were determined. it was established the absence of toxicity of the cherry shoots dry extract in a dose 5000 mg/kg and according to toxicological classification of substances by К.К.Sidorov the cherry shoots dry extract after intragastric administration belong to V toxicity class – practically harmless substances. Cherry shoots dry extract showed the best anti-inflammatory effect in a dose of 100 mg/kg; it was about 36 % on average for 5 hours. Conclusions. Considering results of chemical analysis and determination safety and antiinflammatory activity we can assume that the cherry shoots dry extract can demonstrate analgesic and antiallergic effect, so it is promising for further study in order to create a new effective and safe drugs for use in medical practice. |
URI: | http://dspace.nuph.edu.ua/handle/123456789/14656 |
Appears in Collections: | Наукові публікації кафедри фармакогнозiї та нутриціології |
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