Express method for evaluating the antioxidant activity of medicinal substances and its preparations by polarography.

Abstract

The express method of medicinal substances and preparations (further substances) antiradical and antioxidant activity evaluation by polarography using a microsomal LPO model in vitro is presented in this work. The substances antioxidant properties have been investigated by adding Fe2+-ADP inductive NADPH-dependent (enzymatic) and adding Fe2+-ADP inductive ascorbate-dependent (non-enzymatic) lipid peroxidation (LPO) of microsomes from rat liver in the in vitro system to the polarographic cell, which activity has been recorded at oxygen absorption rate by using standard closed platinum Clark's electrode at 30 °C in 100 mmol of tris-HCl buffer, pH 7.4. It has been shown that it is possible to determine the mechanism of substances antioxidant action, which includes antiradical activity as to lipid and oxygen radicals on enzymatic and ascorbate-dependent intact microsomes LPO models by polarographic method in the in vitro system. It is also possible to investigate the antioxidant action features of medicinal substances, which are determined by their nature, chemical structure peculiarities, concentration and interaction in the pharmaceutical form.