Fetoplacental insufficiency as a reason of offspring oxidative status disturbances

Abstract

The aim of this scientific work was to determine the influence of the experimental fetoplacental insufficiency on the both sex offspring oxidative status during puberty and to estimate the efficiency of base and complex therapy during pregnancy. Materials. The healthy, Vistar mature rat’s females of young (3–4 months) and mature (8–10 months) reproductive age have been used for both sex offspring obtaining. 8 groups for 7 pregnant females in each have been formed: Groups I and II — intact animals of young and mature reproductive age; Group III and IV — females with experimental Fetoplacental insufficiency (FPI) of young and mature reproductive age accordingly; Groups V and VI — young and mature animals with experimental FPI treated by pharmaceutical composition which contains nontoxic active pharmaceutical ingredients of FPI basic therapeutic group — amino acid (L-arginine), dicarbonic acid (succinic acid), vitamins (folic acid) and vasoactive drug (dipyridamole). Experimental animals have received treatment from 11 to 19 day of pregnancy. Groups VII and VIII — young and mature animals with experimental FPI treated by drug of comparison — dipyridamole. The modeling of FPI has been carried out by daily subcutaneous introduction of 50 % tetrachlormethane oil solution in dose of 2 ml/kg of body weight from 12 to 18 day of pregnancy. Animals — offspring have been killed on the 50th day of life (puberty period) by quick decapitation without general anesthesia to avoid negative effects on sex hormones level and antioxidant enzymes systems. Results. The effect of experimental fetoplacental insufficiency in rats of young and mature reproductive age on the oxidative status formation in offspring of both sexes during puberty was determined and the effects of basic and complex therapy during pregnancy were evaluated. It was revealed that fetoplacental insufficiency in the second half of pregnancy leads to the formation in offspring of both sexes, but more pronounced in male offspring, during the puberty, an altered pattern of the antioxidant system enzymatic activity, which is realized in increased levels of both primary and final products of lipoperoxidation and may be the basis for further development of chronic diseases. More pronounced disorders were observed in the offspring of mothers of mature reproductive age, which may be due to the additional influence of involutive processes in the placenta. The use of a vasodilator drug alone and, more effectively in combination, significantly restored the studied parameters.