Experimental study of stress effect on connective tissue metabolism in white rats during subcutaneous adrenaline administration

Abstract

Purpose – to experimentally assess the effect of adrenaline stress on connective tissue metabolism based on morphological studies of white rats’ liver and to determine metabolites-markers of connective tissue (common chondroitin sulfates and individual fractions of glycosaminoglycans) in the blood serum of animals. The studies were conducted in 2018 on white outbred 3-month-old male rats weighing 180-220 grams in Sytenko Institute of Spine and Joint Pathology NAMS of Ukraine”. Animals were divided into 3 groups: the intact group had 5 rats; the control group included 10 animals who were subcutaneously injected with 0.9% NaCl solution daily for 21 days; the experimental group with 10 animals who were administered adrenaline subcutaneously at a dose of 0.5 mg per 100 grams of body weight daily for 21 days. The subcutaneous adrenaline administration to white rats daily for 21 days at a dose of 0.5 mg per 100 g of body weight caused a stress reaction leading to hyperglycemia, fatty hepatodystrophy with the formation of necrosis foci and coarse steatosis on the background of the growth of ALT, AST, and GGT activity by 2.5 and 1.8 times on the 7th day, indicating the cytolytic syndrome with cholestasis. The adrenaline administration to experimental rats for 3 weeks was accompanied by an increase of chondroitin-6 and chondroitin-4-sulfates in the serum content and a decrease in heparan sulfates, which indicated the development of liver fibrosis.