Research on development of gel patch content for correction of skin age-related changes

Abstract

Patches are thin disks on fabric or hydrogel base used as express masks for face skin care. Active agents having anti-aging properties are peptides. One of peptides, especially Acetyl Hexapeptide-8 (argireline) has myorelaxation properties, therefore its incorporation to anti-aging patch composition is vital. The objective of the development is the creation of a safe and easy-to-use preparation to fight against mimic wrinkles in a form of patches with argireline. Subjects of the research are experimental samples of hydrogel patches with argireline. For the identification of an active agent in patch composition a standard Pharmacopoeia determined reaction with ninhydrin for amino acid derivatives was used. Quantitative analysis was conducted using the same reaction with ninhydrin by the absorption spectrophotometry method in visible region. For the hydrogel base samples the organoleptic, physical and chemical as well as structural and mechanical properties research has been conducted. Organoleptic properties researches have shown that the experimental samples have homogeneous surface, are colorless and clear, the odour is peculiar and faint. The samples have positive results of colloidal and thermal stability research; pH value is within acceptable limits (5,0-9,0). The results of structural and mechanical properties research show the availability of acceptable thixotropic properties, strength; prove that the system has a plastic type of flow. Based on preformulation studies an optimal composition of patches with argireline was developed as well as the patches quality factors research was conducted. External appearance, color, odour, colloidal and thermal stability, pH value were determined in accordance with the requirements of Standard of Organization of Ukraine (SOU/СОУ) 24.5-37-103:2004 «Cosmetic gels. Common technical specifications». External appearance – a homogeneous gel-like mass free from foreign impurities; colorless, clear; odour – faint, peculiar; pH 6,3±0,1. Colloidal and thermal stability specifications are met. It has been proven that active agent addition to the patch composition does not have an effect on organoleptic and physical and chemical properties of the research samples. Qualitative analysis was conducted using the Pharmacopoeia determined reaction of ninhydrin solution to amino acids. Its positive effect determined its use for the quantitative analysis by the absorption spectrophotometry method in visible region. It has been determined that active agent release within 30 minutes is more than 80%, and inessential changes in release values are observed from 30 to 60 minutes.