Herbal tea for the treatment of Urinary diseases as Potent diuretic and Anti-inflammatory agent

Abstract

The herbal tea of previously patented composition for the treatment of urinary diseases containing twelve plants (St. John's wort, Wild pansy, Horsetail field, Common heather, Elecampane i.a.) was analyzed for the qualitative composition and quantitative content of phenolic compounds. The diuretic and anti-inflammatory activity of herbal tea thick extract was assessed. The determination of phenolic compounds was carried out by high performance liquid chromatography using standards of phenolic acids and flavonoids. Diuretic activity was checked by the method of Berkhin for the dosage in the range from 100 to 700mg/kg. Level of urination was assessed during 1, 2, and 3 hours of experiment. Edema was induced with 1% carrageenan. The dosage of oral treatment was from 100 to 250mg/kg. Paw volume was checked at time equal to 1, 2, 3, 4, and 5 hours. Several groups of phenolic compounds were detected, and six substances were identified individually. The total content of phenolic compounds was 23.8mg/g, with the content of flavonoids 10.4mg/g and chlorogenic acid 2.7mg/g. The herbal tea showed diuretic activity of 100% growth at the dosage of 200mg/kg. Further increase of dosage led to antidiuretic activity. From the dosage of 500mg/kg we observed the diuretic activity again, which was the highest for the dosage of 700mg/kg showing 271% growth in relation to the control group. The anti inflammatory activity was the highest at the dosage of 200 and 250mg/kg and was equal to 25% in relation to the control group.