Study on identification of active ingredients of combined gel with salvia and willow plant extracts

Abstract

Over recent years, there has been increased attention of scientists around the world to the problem of rheumatic diseases. Rheumatic diseases affect the national economy, lead to significant costs of treatment and reduce the quality of life of patients. The aim of our study was newly developed combined gel with herbal and synthetic ingredients that intended to be used for rheumatic diseases treatment. In the composition of the combined gel as active ingredients dry extracts of leaves of sage and willow bark and methyl salicylate were used. The physicochemical properties of the gel is a homogeneous mass of brown colour with a characteristic smell of methyl salicylate. This emulgel, was made on the basis of hydroxyethyl cellulose, mixes well with water. Chemical reactions and TLC were used to identify the gel components. For the identification of methyl salicylate in the investigated gel, it is recommended to use a reaction with a solution of ferrum (III) chloride, which resulted in a violet coloration. Substances of polyphenolic structure of dry extract of willow leaves and leaves of sage should be confirmed by reactions of

interaction with solutions of potassium hydroxide and concentrated sulfate acid. The method of thin- layer chromatography in comparison with reference standards has proved the presence of both in dry

extracts and in gel derivatives of salicylic acid, mainly similar in structure with salicin and substances of a flavonoid structure, similar in structure to luteolin. Specificity of the test was confirmed by the ability of the proposed solvent system to divide the components of the dosage form. Thus, this solvent system can identify not only substances of the flavonoid structure of plant extracts, but also confirm the presence of other components of the gel - methyl salicylate and methyl parahydroxybenzoate.