Aristolochic acid i determination in Aristolochia clematitis L. raw materials by HPLC metod

Abstract

Aristolochia clematitis has long ago been applied in Asian traditional medicine and demonstrates multidirectional pharmacological activity. Contemporary research has confirmed its expressed antimicrobial activity. Using high performance liquid chromatography (HPLC) we ascertained and determined the content of aristolochic acid 1 in herb and roots of Aristolochia clematitis collected within the blooming period in Kharkiv and Khmelnytskyi Regions, Ukraine. As aristolochic acid I causes nephrotoxic activity of plants, particularly, Aristolochia clematitis, it is quite mandatory to control its content in raw materials. The content of aristolochic acid I in Aristolochia clematitis herb was 0.11±0.01%, in roots its content was 0.14±0.01%. Thus, issuing from the results of our experiment, we concluded that Aristolochia clematitis raw materials may be treated as feasible for the development of drugs, but only for external application. The results of our study may be used for the development of Aristolochia clematitis raw materials standardization parameters and safe application.