The investigation total content of catechins in the blackberry (Rubus plicatus) leaves

Abstract

Blackberry (Rubus fruticosus L.) is a perennial shrub belonging to the rose family (Rosaceae). It is well known for its fruits, which possess significant medicinal, nutritional, and cos- metic value. The genus Rubus represents the largest genus within the Rosaceae family, comprising approximately 700 species. It includes 12 subgenera, many of which contain domesticated as well as wild species. Rubus fruticosus is widely distributed across Europe, Oceania, North and South Amer- ica, and Asia. Considerable variability is observed among species in terms of morphology, flower color, growth habit, and chemical composition. Within this large and diverse genus, numerous wild species and natural hybrids exist, many of which are self-infertile or dioecious, requiring cross-polli- nation for fruit production. The chemical composition of blackberry leaves is complex and character- ized by a broad range of biologically active compounds, including catechins (mainly catechin), or- ganic acids (such as citric and malic acids), hydroxycinnamic acids (such as caffeic and ferulic acids), flavonoids, and ellagitannins. These phytochemicals contribute to the plant’s notable antioxidant, an- timicrobial, and anti-inflammatory properties. Given this background, the present study aimed to de- termine the total catechin content in blackberry leaves using spectrophotometric methods, in order to evaluate their potential contribution to the overall antioxidant capacity of the plant.