The study of protective properties of associated antigens of candida albicans and candida tropicalis

Abstract

Causative agents of candidiasis are yeast fungi of Candida genus, Cryptococcaceae family. The most widespread causative agent of candidiasis is C. albicans, which causes 90 % of surface forms and 50 – 70 % of deep forms. Another causative agent by prevalence is C. tropicalis. The aim of our work was to study the ability of the associated antigens of C. albicans and C. tropicalis to activate together the body’s protective properties against candidal infection. The suspensions of cells of C. albicans and C. tropicalis fungi were subjected to the action of ultrasound, then filtered through a "Vladipore" membrane MFА-МА No.3 providing separation of the biological material with the size of 10 kDa; prefiltration and sterilizing filtration were carried out. The resulting purified antigens of Candida albicans fungal cells with the protein concentration of 3 mg/ml and Candida tropicalis with the protein concentration of 5 mg/ml were mixed in the ratios of 30:70; 70:30; 40:60; 60:40; 50:50. The associated antigens of cells of C. albicans and C. tropicalis fungi in the volume of 0.2 ml were injected to miceintramuscularly. In 14 days the procedure was repeated. The animals of the control group were injected with the sterile 0.9 % isotonic saline solution. In a month and 3 months some groups of the tested animals were infected intra-abdominally with the suspensions of C. albicans fungi in the amount of 20 million of cells and Candida tropicalis in the amount of 60 million of cells in the volume of 1 ml. Afterthat in 14 days the animals were examined and the results were determined. As the result of the research conductedit has been found that application of the associated antigens of C. albicans with the protein concentration of 3 mg/ml and C. tropicalis with the protein concentrationof 5 mg/ml in the ratio of 1:1 (50:50) provide the protective effect in 100 % of mice for 3 months when injected intramuscularly in the volume of 0.2 ml.