Клінічні, біохімічні та імунологічні маркери гіперчутливості в мурчаків під впливом циклорину

Abstract

В статье представлены результаты изучения фармакологической активности нового гомеопатического препарата "Циклорин", содержащего экстракт цикломена европейского, на модели реакции гиперчувствительности немедленного типа с бронхообструктивным синдромом у морских свинок. Установлено, что исследуемый препарат "Циклорин" эффективно предупреждает развитие бронхообструктивного синдрома у сенсибилизированных животных. Циклорин при 21-дневном внутрижелудочном введении в дозе 37 мг/кг оказывает положительное влияние на биохимические, иммунологические и биометрические показатели , не уступая препарату сравнения кетотифену в дозе 0,13 мг/кг. По влиянию на отдельные показатели (интерлейкин -1β,содержание гистамина,уровень циркулирующих иммунных клмплексов) циклорин превосходит препарат сравнения.

The search for new medicinal products for pharmacological correction of allelgic diseases,including bronchial asthma,is an actual problem of contemporary pharmacology. The prospective branch of pharmacotherapy for broncho-obstructive syndrom - is the application of homeopathic medicinal products. With the purpose of creation a new domestic homeopathic medicinal product for curing allergic diseases. "Cyclorine" medicinal product has been developed in the national university of pharmacy on the basis of extract of Cyclamen europaeum, wthich is widely used in homeopathic medicine for curing sinusitis, headache and bronchial asthma. The authors of the article describe results of experimental study of pharmacological activity of"Cyclorine" homeopatic medicinal product at the model of hypersensitive reaction at broncho-obstructive syndrome of guinea pigs. The animals have been divided info four experimental groups:intact control pathology:Cyclorine + ; Ketotefen + broncho-obstructive syndrom. Wthen 21 days, all the animals . but for the group of intact control , are being sensitized wiht normal horse serum, with further introduction of a solving dose of antigen. Cyclorine and Ketotifen are given intragastrically at the dose of 37 mg/kg and 0.13 mg/kg cjrrespondingly,during 21 days, starting with the first day of sensibilization. It was determined that the studied product was effective for prevention of broncho-obstructive syndrome development at sensitezed animals.21--days introduction of Cyclorine at the dose 37 mg/kg had positive impact on the dynamics of biochemical, immunological and biometrical indices. As of impact on leucocytes and eosinophils,titer of heterophil agglutinins,mass coefficients of internals (lungs, thymus,and lien) Ceclorine was is good as cjmparative substance Ketotifen. As of impact on certain indices ( interleukin-1β, histamine, level of circulating immune complexes),Ceclorine was better that the comparative substace. Effictiveness of the product was due stabilization of membranes of mast cells and impact on histamine release.