Ізолювання піразидолу з біологічного матеріалу амфіпротонними розчинниками

Abstract

Вивчена роздільна здатність відносно піразидолу загальноприйнятих у судово-токсикологічному аналізі методів ізолювання лікарських речовин настоюванням з амфіпротонними розчинниками.

The resolution of the isolation methods of medicinal substances generally accepted in forensic-toxicological analysis by infusion with amphiprotonic solvents has been studied with regard to pyrazidol. It has been shown that methods by O.O.Vasilyeva allowed to separate 7.14+0.83%, by Stas-Otto — 14.64+1.24%, by V.F.Kramarenko — 12.52+0.97% of pyrazidol. Detection and the quantitative determination of pyrazidol in the extracts was carried out by the methods of Thin Layer Chromatography, UV-spectroscopy, extraction photometry after the additional purification of the extracts from concomitant admixtures by means of back extraction and TLC. The reaction of ionic associate formation of pyrazidol with methyl orange, the acidic azodye, was used for the extraction photometry determination. Absorbance of the coloured solutions was subMected to the law of Bouguer-Lambert-Behr within the concentration range of 5-120 mg of pyrazidol in 14 ml of the final volume. The relative error of the quantitative determination did not exceed 2.8%. The results obtained may be used for forensic-toxicological examinations of the biological material in mortal pyrazidol poisonings.