Please use this identifier to cite or link to this item: http://dspace.nuph.edu.ua/handle/123456789/19954
Title: LC-MS/MS method development and validation for the determination of cardiazol in human plasma
Authors: Drapak, I. V.
Zimenkovsky, B. S.
Perekhoda, L. O.
Kovalenko, S. M.
Logoyda, L.
Драпак, І. В.
Зіменковський, Б. С.
Перехода, Л. О.
Коваленко, С. М.
Логойда, Л.
Keywords: LC-MS/MS;Cardiazol;Human plasma;Validation;Pharmacokinetic studies
Issue Date: 2019
Publisher: International Journal of Applied Pharmaceutics
Bibliographic description (Ukraine): LC-MS/MS method development and validation for the determination of cardiazol in human plasma / I. V. Drapak, B. S. Zimenkovsky, L. O. Perekhoda, S. M. Kovalenko, L. Logoyda // Int. J. App. Pharm. – 2019. – Vol. 11. – Р. 380-385.
Abstract: The main purpose of this study was to develop a simple, precise, rapid and accurate method for the quantification of cardiazol in human plasma.
Description: The total chromatographic run time was 2.5 min and the elution of cardiazol and IS (difenoconazole) occurred at ~2.15 and 1.98 min, respectively. A linear response function was established at 1-100 ng/ml for cardiazol and difenoconazole in human plasma. The % mean recovery for cardiazol in LQC, MQC and HQC was 102.8 %, 100.3 % and 95.9 %. The lowest concentration with the RSD<20% was taken as LLOQ and was found to be 1.10 ng/ml for cardiazol. The % accuracy of LLOQ samples prepared with the different biological matrix lots was found 109.7 %, which were found within the range of 80.00-120.00 % for the seven different plasma lots. % CV for LLOQ samples was observed as 11.9 %, which are within 20.00% of the acceptance criteria. The within-run coefficients of variation ranged between 0.311 % and 0.601 % for cardiazol. The within-run percentages of nominal concentrations ranged between 99.80 % and 100.41 % for cardiazol. The between-run coefficients of variation ranged between 0.332 % and 0.615 % for cardiazol. The between-run percentages of nominal concentrations ranged between 98.18 % and 101.21 % for cardiazol.
URI: http://dspace.nuph.edu.ua/handle/123456789/19954
Appears in Collections:Наукові публікації кафедри фармацевтичної хімії

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